Immunology Profiles: T Cells and Innate Lymphoid Cells in Mucosal Immunity

This is a sponsored article by STEMCELL Technologies.

Immunology Profiles is a venue where Immunologists around the world tell their stories, discuss their research, and voice their thoughts and opinions on current topics in immunology. From research institutes such as the National Institutes of Health and Yale School of Medicine, and they share their research on autoimmunity, immune tolerance and more and why they are using EasySep™ to isolate their cells. Amongst them, Kyle Burrows is an immunologist with a passion for CD4+ T cells and innate lymhoid cells (ILCs) in mucosal immune regulation. Watch Kyle tell his story and discuss his research in this interview.

Kyle received his BSc from the University of British Columbia. His current graduate research in Dr. Colby Zaph’s lab is aimed to investigate the molecular mechanisms that regulate CD4+ T cell subsets and ILCs in mucosal sites. Dr. Colby Zaph is now a Professor at the Biomedicine Discovery Institute, Monash University, and the research at his lab is focused on defining the cellular and molecular mechanisms controlling mucosal inflammation in the intestine and the lung. The Zaph lab utilizes animal models to investigate how perturbations in these mechanisms can result in diseases such as food allergies, inflammatory bowel disease (IBD), and asthma.

Kyle Burrows, BScPhD Candidate, Dr. Colby Zaph’s Lab:

Over the past 5 years, ILCs have been defined as a new arm of innate immunity, and have gained tremendous popularity. ILCs are characterized by the lack of cell surface markers that define other immune cell lineages (Lin), making them difficult to isolate and analyze (Artis and Spits, 2015, Nature). These cells do not possess any antigen specific receptors; however, like T cells, they commonly express CD25 and CD127.ILCs can be divided into 3 major groups: T-bet+ ILC1, which express IFN-γ, GATA-3+ ILC2, which secrete IL-4, IL-5 and IL-13, and RORγt+ ILC3, which can secrete IL-17A (Artis and Spits, 2015, Nature). These groups of ILCs can be distinguished by their expression of different cytokine receptors. Recently, the role of ILCs have been highlighted in mucosal immunity: ILC2s play a critical role in the development of allergy (Halim et al, 2014, Immunity) and ILC3s are involved in the pathogenesis of inflammatory bowel disease (Eken et al, 2014, Musocal Immunology). To provide a quick reference for immunologists interested in studying ILCs like Kyle, STEMCELL Technologies has partnered with Nature to create and distribute copies of Innate Lymphoid Cell wallchart.

See more from Kyle Burrows at Immunology Profiles

Immunologists like Kyle Burrows rely on cell isolation technologies to obtain highly purified cells for downstream experiments. More immunologists, including those featured in Immunology Profiles, are now choosing EasySep™ to help them isolate their cells. EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system for fast and easy isolation of highly purified cell populations that are immediately ready for downstream applications. This powerful and versatile immunomagnetic cell separation platform enables positive selection, negative selection, or cell depletion from virtually any type of sample, including splenocytes, lymph nodes, PBMCs, whole blood, cord blood, bone marrow and leukapheresis samples (Leuko Paks). EasySep™ can be used to isolate cells in as little as 8 minutes from a variety of species, including human, mouse, non-human primate, rat, rabbit, cow and pig. Immunologists needing more specialized and rare immune cells can pre-enrich cells prior to flow sorting to save time, reagents and cost.

Watch this short video to learn more:

Visit Immunology Profiles to see why immunologists choose EasySep™ over other cell separation platforms. Here is why Kyle uses this technology to isolate mouse immune cells, including CD4+ T cells:

EasySep™ helps my research because it is very quick and easy, so it is a great way to isolate cells fast in order to get a pure population that has great viability. We do not have to worry about any of the cells getting lost.” – Kyle Burrows

Are you ready to try EasySep™ in your own lab? Request a sample.

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